Understanding the dynamics of biochemical networks is important for understanding life at the systems level and has practical implications for Medicine, Engineering, Biology, and Chemistry. The complex network of hemostasis consists of over 100 coupled reactions, and has the death-defying function of regulating blood coagulation. Research in my laboratory focuses on three problems related to blood coagulation: i) understanding the biophysical mechanisms by which blood clots form and degrade, ii) controlling these processes to eliminate unwanted coagulation, and iii) using coagulation as a scaffold for delivering therapeutics to diseased vasculature. This research lies at the interface of Chemical Biology, Bioengineering and Medicine. The techniques we use include microfluidics, reconstituted protein systems, biomaterials synthesis, numerical simulations, and disease models of coagulation and atherosclerosis.
Current Lab Members:
Steve Wooksuk Hur
Ju Hun Yeon
Cameron Kelvin Chan
C.J. Kastrup, M. Nahrendorf, J.L. Figueiredo, H. Lee, S. Kambhampati, T. Lee, S.W. Cho, R. Gorbatov, Y. Iwamoto, T.T. Dang, P. Dutta, H.H. Yeon, H. Cheng, C.D. Pritchard, A.J. Vegas, C.D. Siegel, S. MacDougall, M. Okonkwo, J.R. Stone, A.J. Coury, R. Weissleder, R. Langer, D.G. Anderson, “Painting Blood Vessels and Atherosclerotic Plaques with an Adhesive Drug Depot”, Proc Natl Acad Sci USA, In Press (2012)
A. Schroeder, M.S. Goldberg, C.J. Kastrup, Y. Wang, B.J. Jospeh, C.G. Levins, R. Langer, D.G. Anderson, Remotely-activated Protein-Producing Nanoparticles, Nano Letters, 2012 (12):2685-2689.
H. Cheng, M. Byrska-Bishop, C.T. Zhang, C.J. Kastrup, N.S. Hwang, W.W. Lee, X. Xu, M.P. Nahrendorf, R. Langer, D.G. Anderson,” Conjugation Tuned Peptides on Stem Cell Membranes for Artificial Cell Rolling”, Biomaterials, 2012 (33): 5004-12.
D. Chen, K. Love, Y. Chen, A. Eltoukhy, C.J. Kastrup, G. Sahay, A. Jeon, D. Yizhou, K. Whitehead, D.G. Anderson, Rapid Discovery of Potent siRNA-Lipid-Nanoparticles Enabled by Controlled Microfluidic Formulation, Journal of the American Chemical Society, 2012 (134):6948-6951.
H. Cheng, C.J. Kastrup, R. Ramanathan, D.J. Siegwart, M. Ma, S.R. Bogatyrev, Q. Xu, K.A. Whitehead, R. Langer, D.G. Anderson. Nanoparticulate cellular patches for cell-mediated tumoritropic delivery. ACS Nano 4 (2) 625-31. (2010)
C.J. Kastrup, J.Q. Boedicker, A.P. Pomerantsev, M. Moayeri, Y. Bian, R.R. Pompano, T.R. Kline, P. Sylvestre, F. Shen, S.H. Leppla, W.J.Tang, R.F. Ismagilov. Spatial localization of bacteria controls coagulation of human blood by ”quorum acting”. Nature Chemical Biology. 4 (12) 742-50. (2008)
C.J. Kastrup, M.K. Runyon, E.M. Lucchetta, J.M. Price, R.F. Ismagilov. Using chemistry and microfluidics to understand the spatial dynamics of complex biological networks. Accounts of Chemical Research. 41 (4) 549-58. (2008)
F. Shen, C.J. Kastrup, Y. Liu, R.F. Ismagilov. Threshold response of initiation of blood coagulation by tissue factor in patterned microfluidic capillaries is controlled by shear rate. Arteriosclerosis Thromobosis and Vascular Biology. 28 (11) 2035-41. (2008)
C.J. Kastrup, M.K. Runyon, E.M. Lucchetta, J.M. Price, R.F. Ismagilov. Modular chemical mechanism predicts spatiotemporal dynamics of initiation in the complex network of hemostasis. Proceedings of the National Academy of Sciences USA 103 (43) 15747-52. (2006)