Franck Duong
Professor
Biochemistry and Molecular Biology
Faculty of Medicine
Education
University of Marseilles, France, 1990, BSc
University of Marseilles, France, 1994, PhD
Dartmouth Medical School, USA, 1998, Postdoctoral Fellow
CNRS-University of Paris-Sud, France, 2004, Principal Investigator
Contact
Office: Life Sciences Centre, 5407
Office Phone: (604) 822–5975
Office Phone 2: (604) 822–5245
E-mail: fduong@mail.ubc.ca
Website: https://theduongresearchgroup.com/
Investigating Membrane Protein Structure, Function and Interactions using the Peptidisc
Membrane proteins represent ~60% of medical drug targets and encompass 1/5th of the human proteome; yet these proteins are vastly under-represented in structure and interaction databases. This discrepancy is due to biochemical and biophysical studies requiring proteins in a water-soluble state, while membrane proteins are naturally sequestered in a hydrophobic lipid environment. In order to render membrane proteins into a soluble state, that is amenable for their study, researchers generally use detergents to extract and purify these proteins. However, these surfactants have many undesired effects on protein structure, function and downstream analysis. Work from our group and others has focused on developing nanotechnology-based reconstitution systems to try replacing the natural lipid bilayer while maintaining water-solubility. These membrane mimetics, either protein-based scaffolds or synthetic polymers, have been around for a decade, yet their utilities did not reach the expected potential due to the optimization and adaptation required for each membrane protein system. Recently, our laboratory developed a “one-size-fits-all” formulation known as the Peptidisc— the Peptidisc is made by multiple copies of an amphipathic peptide that spontaneously associate around transmembrane domains of proteins upon removal of detergent. The peptide number adapts spontaneously to fit the size and shape of the protein, allowing for minimal reconstitution optimization. The end result is a membrane protein that is stable, free of detergent effects, and soluble in aqueous solution. The Peptidisc is a new tool that we hope will allow more researchers, including those who are not expert biochemists, to study membrane proteins. This will yield a better understanding of the structure and function of the cellular membrane as it interacts with the environment. Since the approach is both simple and easy to apply, more membrane proteins may now be included in high-throughput searches for potential new drugs that may help treat various medical conditions.
Comprehensive List
Selected Publications
- Sensitive Profiling of Mouse Liver Membrane Proteome Dysregulation Following a High‐Fat and Alcohol Diet Treatment
F Antony, Z Brough, M Orangi, M Al‐Seragi, H Aoki, M Babu, …
Proteomics 24 (23-24), e202300599
2024 - From bottom-up to cell surface proteomics: detergents or no detergents, that is the question
Z Brough, Z Zhao, F Duong van Hoa
Biochemical Society Transactions
3, 2024 - Affinity Purification of Membrane β-Barrel Proteins via Biotin-Tagged Peptidiscs
Z Zhao, JW Young, F Duong van hoa
Transmembrane β-Barrel Proteins: Methods and Protocols, 147-158
2024 - Capture of the mouse organ membrane proteome specificity in peptidisc libraries
F Antony, Z Brough, Z Zhao, F Duong van Hoa
Journal of Proteome Research 23 (2), 857-867
6, 2024 - YibN, a bona fide interactor of YidC with implications in membrane protein insertion and membrane lipid production
Z Zhao, N Yamamoto, J Young, N Solis, A Fong, M Al-Seragi, S Kim, …
bioRxiv, 2024.12. 13.628402
2024 - Membrane mimetic thermal proteome profiling (MM-TPP) towards mapping membrane protein-ligand dynamics
RS Jandu, M Al-Seragi, H Aoki, M Babu, FVH Duong
bioRxiv, 2024.11. 02.621677
2024 - Profiling the organ membrane proteome dysregulation in the context of liver disease
F Antony, Z Brough, M Orangi, H Aoki, M Babu, F Duong van Hoa
bioRxiv, 2024.04. 05.588300
1, 2024 - Sequestering agents, kits therefor, and methods of using sequestering agents and kits therefor
M Carlson, F Duong
US Patent App. 17/972,170
2023 - A dual detergent strategy to capture a bacterial outer membrane proteome in peptidiscs for characterization by mass spectrometry and binding assays
JW Young, Z Zhao, IS Wason, F Duong van Hoa
Journal of Proteome Research 22 (5), 1537-1545
8, 2022 - Sequestering agents, kits therefor, and methods of using sequestering agents and kits therefore
M Carlson, F Duong
US Patent 11,479,485
2022 - Development of a method combining peptidiscs and proteomics to identify, stabilize, and purify a detergent-sensitive membrane protein assembly
JW Young, IS Wason, Z Zhao, S Kim, H Aoki, S Phanse, DG Rattray, …
Journal of proteome research 21 (7), 1748-1758
17, 2022 - Structural dynamics of the functional nonameric type III translocase export gate
B Yuan, AG Portaliou, R Parakra, JH Smit, J Wald, Y Li, B Srinivasu, …
Journal of Molecular Biology 433 (21), 167188
8, 2021 - Nanodisc-based proteomics identify Caj1 as an Hsp40 with affinity for phosphatidic acid lipids
XX Zhang, JW Young, LJ Foster, F Duong
Journal of Proteome Research 20 (10), 4831-4839
5, 2021 - Hydrophobic biomolecule stabilizing scaffold peptides and methods of making and using same
F Duong, M Carlson, H Dhupar
US Patent App. 17/059,854
2021 - Structural and unctional diversity calls for a new classification of ABC transporters
C Thomas, SG Aller, K Beis, EP Carpenter, G Chang, L Chen, E Dassa, …
FEBS letters 594 (23), 3767-3775
230, 2020 - Functional self-nonamerization of the Type III translocase chaperone/exported protein receptor
B Yuan, AG Portaliou, Y Li, J Wald, JH Smit, HS Dhupar, BY Srinivasu, …
bioRxiv, 2020.11. 20.391094
1, 2020 - Structural insight into the Staphylococcus aureus ATP-driven exporter of virulent peptide toxins
N Zeytuni, SW Dickey, J Hu, HT Chou, LJ Worrall, JAN Alexander, …
Science Advances 6 (40), eabb8219
17, 2020 - His-tagged peptidiscs enable affinity purification of the membrane proteome for downstream mass spectrometry analysis
JW Young, IS Wason, Z Zhao, DG Rattray, LJ Foster, F Duong Van Hoa
Journal of proteome research 19 (7), 2553-2562
17, 2020 - New approach for membrane protein reconstitution into peptidiscs and basis for their adaptability to different proteins
G Angiulli, HS Dhupar, H Suzuki, IS Wason, F Duong Van Hoa, T Walz
Elife 9, e53530
90, 2020 - Profiling the E. Coli Membrane Interactome Captured in Peptidisc Libraries
I Wason, IS Wason, G Stacey, J Young, M Carlson, Z Zhao, DG Rattray, …
PROTEIN SCIENCE 28, 70-71
2019 - Discovering Novel Antibodies Against Peptidisc Stabalized Membrane Proteins
J Saville, F Duong, K Zhao
Protein Science 28, 51-52
2019 - Profiling the Escherichia coli membrane protein interactome captured in Peptidisc libraries
ML Carlson, RG Stacey, JW Young, IS Wason, Z Zhao, DG Rattray, …
Elife 8, e46615
73, 2019 - Investigating the stability of the SecA–SecYEG complex during protein translocation across the bacterial membrane
J Young, F Duong
Journal of Biological Chemistry 294 (10), 3577-3587
11, 2019 - Selectively targeting the dimerization interface of human androgen receptor with small-molecules to treat castration-resistant prostate cancer
K Dalal, F Ban, H Li, H Morin, M Roshan-Moniri, KJ Tam, A Shepherd, …
Cancer letters 437, 35-43
62, 2018 - The Peptidisc, a simple method for stabilizing membrane proteins in detergent-free solution
ML Carlson, JW Young, Z Zhao, L Fabre, D Jun, J Li, J Li, HS Dhupar, …
Elife 7, e34085