Franck Duong
Professor
Biochemistry and Molecular Biology
Faculty of Medicine
Education
University of Marseilles, France, 1990, BSc
University of Marseilles, France, 1994, PhD
Dartmouth Medical School, USA, 1998, Postdoctoral Fellow
CNRS-University of Paris-Sud, France, 2004, Principal Investigator
Contact
Office: Life Sciences Centre, 5407
Office Phone: (604) 822–5975
Office Phone 2: (604) 822–5245
E-mail: fduong@mail.ubc.ca
Website: https://theduongresearchgroup.com/
Dr. Franck Duong’s research centers on the structural and functional characterization of membrane proteins, with a strong focus on developing tools for their solubilization and analysis under native-like conditions. His lab pioneered the Peptidisc—a self-assembling, amphipathic peptide scaffold that stabilizes integral membrane proteins in aqueous solution without detergents. Unlike traditional mimetics like detergents or synthetic nanodiscs, the Peptidisc preserves protein-lipid interactions and simplifies reconstitution directly from biological membranes. This enables downstream applications in structural biology, proteomics, and interaction mapping.
Current work in the Duong Lab integrates the Peptidisc with mass spectrometry-based proteomics to study membrane protein complexes at scale, including:
- Characterization of large, multi-subunit assemblies such as bacterial secretion systems and eukaryotic transporters.
- Proteomic profiling of tissue-specific membrane proteomes from mouse organs using detergent-free extraction to preserve labile complexes.
- Investigating native protein-lipid interactions by co-purifying membrane proteins with bound lipids for functional studies.
The lab also develops nanobody-based technologies for membrane protein targeting. Using Peptidisc-stabilized proteins as platforms, they’ve introduced strategies for nanobody multimerization and multispecificity, with potential applications in diagnostics, structural studies, and therapeutics. Overall, the Duong Lab advances membrane protein biochemistry through scalable methods for solubilization, stabilization, and functional analysis, while fostering collaborations across structural biology, pharmacology, and systems biology.
Comprehensive List
Selected Publications
- Sensitive Profiling of Mouse Liver Membrane Proteome Dysregulation Following a High‐Fat and Alcohol Diet Treatment
F Antony, Z Brough, M Orangi, M Al‐Seragi, H Aoki, M Babu, …
Proteomics 24 (23-24), e202300599
2024 - From bottom-up to cell surface proteomics: detergents or no detergents, that is the question
Z Brough, Z Zhao, F Duong van Hoa
Biochemical Society Transactions
3, 2024 - Affinity Purification of Membrane β-Barrel Proteins via Biotin-Tagged Peptidiscs
Z Zhao, JW Young, F Duong van hoa
Transmembrane β-Barrel Proteins: Methods and Protocols, 147-158
2024 - Capture of the mouse organ membrane proteome specificity in peptidisc libraries
F Antony, Z Brough, Z Zhao, F Duong van Hoa
Journal of Proteome Research 23 (2), 857-867
6, 2024 - YibN, a bona fide interactor of YidC with implications in membrane protein insertion and membrane lipid production
Z Zhao, N Yamamoto, J Young, N Solis, A Fong, M Al-Seragi, S Kim, …
bioRxiv, 2024.12. 13.628402
2024 - Membrane mimetic thermal proteome profiling (MM-TPP) towards mapping membrane protein-ligand dynamics
RS Jandu, M Al-Seragi, H Aoki, M Babu, FVH Duong
bioRxiv, 2024.11. 02.621677
2024 - Profiling the organ membrane proteome dysregulation in the context of liver disease
F Antony, Z Brough, M Orangi, H Aoki, M Babu, F Duong van Hoa
bioRxiv, 2024.04. 05.588300
1, 2024 - Sequestering agents, kits therefor, and methods of using sequestering agents and kits therefor
M Carlson, F Duong
US Patent App. 17/972,170
2023 - A dual detergent strategy to capture a bacterial outer membrane proteome in peptidiscs for characterization by mass spectrometry and binding assays
JW Young, Z Zhao, IS Wason, F Duong van Hoa
Journal of Proteome Research 22 (5), 1537-1545
8, 2022 - Sequestering agents, kits therefor, and methods of using sequestering agents and kits therefore
M Carlson, F Duong
US Patent 11,479,485
2022 - Development of a method combining peptidiscs and proteomics to identify, stabilize, and purify a detergent-sensitive membrane protein assembly
JW Young, IS Wason, Z Zhao, S Kim, H Aoki, S Phanse, DG Rattray, …
Journal of proteome research 21 (7), 1748-1758
17, 2022 - Structural dynamics of the functional nonameric type III translocase export gate
B Yuan, AG Portaliou, R Parakra, JH Smit, J Wald, Y Li, B Srinivasu, …
Journal of Molecular Biology 433 (21), 167188
8, 2021 - Nanodisc-based proteomics identify Caj1 as an Hsp40 with affinity for phosphatidic acid lipids
XX Zhang, JW Young, LJ Foster, F Duong
Journal of Proteome Research 20 (10), 4831-4839
5, 2021 - Hydrophobic biomolecule stabilizing scaffold peptides and methods of making and using same
F Duong, M Carlson, H Dhupar
US Patent App. 17/059,854
2021 - Structural and unctional diversity calls for a new classification of ABC transporters
C Thomas, SG Aller, K Beis, EP Carpenter, G Chang, L Chen, E Dassa, …
FEBS letters 594 (23), 3767-3775
230, 2020 - Functional self-nonamerization of the Type III translocase chaperone/exported protein receptor
B Yuan, AG Portaliou, Y Li, J Wald, JH Smit, HS Dhupar, BY Srinivasu, …
bioRxiv, 2020.11. 20.391094
1, 2020 - Structural insight into the Staphylococcus aureus ATP-driven exporter of virulent peptide toxins
N Zeytuni, SW Dickey, J Hu, HT Chou, LJ Worrall, JAN Alexander, …
Science Advances 6 (40), eabb8219
17, 2020 - His-tagged peptidiscs enable affinity purification of the membrane proteome for downstream mass spectrometry analysis
JW Young, IS Wason, Z Zhao, DG Rattray, LJ Foster, F Duong Van Hoa
Journal of proteome research 19 (7), 2553-2562
17, 2020 - New approach for membrane protein reconstitution into peptidiscs and basis for their adaptability to different proteins
G Angiulli, HS Dhupar, H Suzuki, IS Wason, F Duong Van Hoa, T Walz
Elife 9, e53530
90, 2020 - Profiling the E. Coli Membrane Interactome Captured in Peptidisc Libraries
I Wason, IS Wason, G Stacey, J Young, M Carlson, Z Zhao, DG Rattray, …
PROTEIN SCIENCE 28, 70-71
2019 - Discovering Novel Antibodies Against Peptidisc Stabalized Membrane Proteins
J Saville, F Duong, K Zhao
Protein Science 28, 51-52
2019 - Profiling the Escherichia coli membrane protein interactome captured in Peptidisc libraries
ML Carlson, RG Stacey, JW Young, IS Wason, Z Zhao, DG Rattray, …
Elife 8, e46615
73, 2019 - Investigating the stability of the SecA–SecYEG complex during protein translocation across the bacterial membrane
J Young, F Duong
Journal of Biological Chemistry 294 (10), 3577-3587
11, 2019 - Selectively targeting the dimerization interface of human androgen receptor with small-molecules to treat castration-resistant prostate cancer
K Dalal, F Ban, H Li, H Morin, M Roshan-Moniri, KJ Tam, A Shepherd, …
Cancer letters 437, 35-43
62, 2018 - The Peptidisc, a simple method for stabilizing membrane proteins in detergent-free solution
ML Carlson, JW Young, Z Zhao, L Fabre, D Jun, J Li, J Li, HS Dhupar, …
Elife 7, e34085