Our group utilizes biochemical engineering to solve problems related to hemostasis and thrombosis. We investigate, utilize, and mimic the biochemistry and biophysical dynamics of blood coagulation to create innovative materials that perform new functions inside of blood vessels, and work to develop novel treatments for severe hemorrhage. We use a variety of biochemical assays, genetic engineering tools, blood samples, biomaterial synthesis, microfluidics, imaging, and small and large animal models to answer the following research questions.
Lab Research Questions
What new technologies are needed to halt bleeding in the most severe cases of hemorrhage?
Can platelets be genetically modified to enhance their hemostatic activity, or targeted to treat Alzheimer’s Disease?
Can clot stability be altered to treat hemorrhage or thrombosis?
Do soil and microbes in wounds influence bleeding?
I am interested in recruiting new students for September 2019.
Current Lab Members:
Lih Jiin Juang
Jerry Leung, Co-supervised in Cullis Lab
Steve Wooksuk Hur
Baylis JR, Lee MM, St. John AE, Wang X, Simonson E, Cau M, Kazerooni A, Gusti V, Statz ML, Yoon JSJ, Liggins RT, White NJ, Kastrup CJ, “Topical tranexamic acid is more effective when formulated with self-propelling particles”, J Thrombosis Haemostasis Accepted, 2019-05.
Hur WS, Warner H, Machan L, Kastrup CJ, “Coagulation Factors XIII-A and XIII-A* Decrease In Some DVT Patients Following Catheter-directed Thrombolysis”, Blood Coagulation and Fibrinolysis, Accepted, 2019-1.
Hur WS, Mazinani N, Lu XJD, Yefet LS, Byrnes JR, Ho L, Yeon JH, Filipenko S, Wolberg AS, Jefferies WA, Kastrup CJ, “Coagulation Factor XIIIa can Crosslink Amyloid β into Dimers and Oligomers and to Blood Proteins”, Journal of Biological Chemistry(SA), Accepted and In Press, 2019-01.
Novakowski SK, Jiang K, Prakash G, Kastrup CJ., “Delivery of mRNA to Platelets using Lipid Nanoparticles”, Scientific Reports, 2019: 9, 552.
R.L.R. Carter, K. Talbot, W.S. Hur, S.C. Meixner, J.G. Van Der Guten, D.T. Holmes, H.C.F. Cote, C.J. Kastrup, T.W. Smith, A.Y.Y. Lee, E.L.J. Pryzdial, “Rivaroxaban and Apixaban Induce Clotting Factor Xa Fibrinolytic Activity”, J Thromb Haemost 2018; 16(11): 2276-2288.
Chan V, Sarkari M, Sunderland R, St. John AE, White NJ, Kastrup CJ., “Platelets loaded with liposome‐encapsulated thrombin have increased coagulability”, J Thromb Haemost 2018; 16: 1226–1235.
R.R. Pompano, A.H. Chiang, C.J. Kastrup, R.F. Ismagilov, “Conceptual and experimental tools to understand spatial effects and transport phenomena in nonlinear biochemical networks illustrated with patchy switching”, Annual Review of Biochemistry, 2017: 86.
J.H. Yeon, N. Mazinani, T.S. Schlappi, K.Y.T. Chan, J.R. Baylis, S.A. Smith, A.J. Donovan, D. Kudela, G.D. Stucky, Y. Liu, J.H. Morrissey, C.J. Kastrup., “Localization of short-chain polyphosphate enhances its ability to clot flowing blood plasma”, Scientific Reports, 2017: 7: 42119.
E.L. Pryzdial, S.C. Meixner, K. Talbot, L.J. Eltringham-Smith, J.R. Baylis, F.M. Lee, C.J. Kastrup, W.F. Sheffield, “Thrombolysis by chemically modified coagulation factor Xa”, Journal of Thrombosis and Haemostasis, 2016.
J.R. Baylis, A. Finkelstein-Kulka, L. Macias-Valle, J. Manji, M. Lee, E. Levchenko, C. Okpaleke, S. Al-Salihi, A. Javer, C.J. Kastrup, “Rapid hemostasis in a sheep model using particles that propel thrombin and tranexamic acid”, The Laryngoscope, 2016.
J.R. Baylis, K.Y.T. Chan, C.J. Kastrup, “Halting hemorrhage with self-propelling particles and local drug delivery”, Thrombosis Research, 2016: 141: S26-S39.
K.Y.T. Chan, C.Y. Zhao, E.M.J. Siren, J.C.Y. Chan, J. Boschman, C.J. Kastrup, “Adhesion of blood clots can be enhanced when copolymerized with a macromer that is cross-linked by coagulation factor XIIIa”, Biomacromolecules, 2016: 17(6): 248–2252.
J.R. Baylis, A.E. St. John, X. Wang, E.B. Lim, M.L. Statz, E. Simonson, S.A. Stern, R.T. Liggins, N.J. White, C.J. Kastrup, “Self-propelled dressings containing thrombin and tranexamic acid improve short-term survival in a swine model of lethal junctional hemorrhage”, Shock, 2016: 46: 123-128.
W.S. Hur, N. Mazinani, X.J.D. Lu, H.M. Britton, J.R. Byrnes, A.S. Wolberg, C.J. Kastrup, “Coagulation Factor XIIIa is Inactivated by Plasmin”, Blood, 2015: 126(20): 2329-2337.
Chan, S.K. Novakowski, S. Law, C. Klein-Bosgoed, C.J. Kastrup, “Controlled transcription of exogenous mRNA in platelets using protocells”, Angewandte Chemie, 2015: 54(46):13590–13593.
J.R. Baylis, J.H. Yeon, M.H. Thomson, A. Kazerooni, X Wang, A.E. St. John, E.B. Lim, D. Chien, A. Lee, J.Q. Zhang, J.M. Piret, L.S. Machan, T.F. Burke, H.J. White, C.J. Kastrup, “Self-Propelling Particles that Transport Cargo Through Flowing Blood and Halt Hemorrhaging”, Science Advances, 2015: 1(9), e1500379.
J.H. Yeon, K.Y.T. Chan, T.C. Wong, K. Chan, M. R. Sutherland, R.F. Ismagilov, E.L.G. Pryzdial, C.J. Kastrup, “A biochemical network can control formation of a synthetic material by sensing numerous specific stimuli”, Scientific Reports, 2015: (5):10274.
Kretschmer, E. Reiner, G. Hu, N. Tam, D.L. Oliveira, M. Caza, J.H. Yeon, J. Kim, C.J. Kastrup, W.H. Jung. J.W. Kronstad, “Defects in phosphate acquisition and storage influence the virulence of Cryptococcus neoformans”, Infection and Immunity, 2014: 2697-2712.
C.J. Kastrup, M. Nahrendorf, J.L. Figueiredo, H. Lee, S. Kambhampati, T. Lee, S.W. Cho, R. Gorbatov, Y. Iwamoto, T.T. Dang, P. Dutta, J.H. Yeon, H. Cheng, C.D. Pritchard, A.J. Vegas, C.D. Siegel, S. MacDougall, M. Okonkwo, J.R. Stone, A.J. Coury, R. Weissleder, R. Langer, D.G. Anderson, “Painting Blood Vessels and Atherosclerotic Plaques with an Adhesive Drug Depot”, Proceedings of the National Academy of Sciences. 2012 (109): 21444-9.
Schroeder, M.S. Goldberg, C.J. Kastrup, Y. Wang, B.J. Joseph, C.G. Levins, R. Langer, D.G. Anderson, “ Remotely-activated Protein-Producing Nanoparticles”, Nano Letters, 2012 (12):2685-2689.