Associate Professor, Wine Research Centre,
Faculty of Land and Food Systems
Associate Member of the Michael Smith Laboratories
Canada Research Chair and MSFH
phone: 6048275744
fax: 6048225143
Food, Nutrition & Health Bldg, Room 325
2205 East Mall
Vancouver, BC V6T 1Z4
Canada

Dr. Measday is recruiting grads for September 2021.

The goal of my lab is to understand how mobile genetic elements are targeted into the genome using budding yeast as a model system. Retrotransposons are repetitive DNA elements in the genome that can replicate and insert a new copy into the genome via an RNA intermediate.  The Ty1 retrotransposon of Saccharomyces cerevisiae (S. cerevisiae) is an excellent model system to study retroviral integration because the process of Ty1 retrotransposition resembles that of retroviruses including human immunodeficiency virus type 1 (HIV-1) which is the cause of acquired immunodeficiency syndrome (AIDS).  The integration of retroviral DNA into genomes is not a random process but requires targeting by the interaction of retroviral integrase (IN) with host proteins to regions of the genome that will cause minimal damage to the host. The Ty1 element inserts upstream of genes transcribed by RNA Polymerase (Pol) III such as transfer RNA (tRNA) genes.

RNA Polymerase III subunits are host factors that help target Ty1-IN and its associated complementary DNA (Ty1 cDNA) upstream of tRNA genes the S. cerevisiae genome. Another critical feature of Ty1 insertion is that it requires nucleosomes and inserts twice per nucleosome. We have established a collaboration with an RNA Polymerase III expert, Dr. Hung-Ta Chen, at Institute of Molecular Biology, Academic Sinica, Taipei, Taiwan to identify RNA Pol III subunits needed for Ty1 insertion into the genome.

Current Projects:

•    Identification of RNA Pol III subunits critical for Ty1-Integrase targeting in Saccharomyces cerevisiae
•    Whole genome-mapping of Ty1 element insertion in RNA Pol III mutant strains
•    Analysis of structural chromosome complexes required for Ty1 insertion

Publication link: https://measday-lab.landfood.ubc.ca/publications/

McCarthy, G. C., Morgan, S. C., Martiniuk, J. T., Newman, B.L., Measday, V. and Durall, D. M. (2020) “An indigenous Saccharomyces uvarum population with high genetic diversity dominates uninoculated Chardonnay fermentations at a Canadian Winery”, in revision for PLOS ONE

Cheng, E.*, Martiniuk, J. T.*, Hamilton, J., McCarthy, G., Castellarin, S. D. and Measday, V. (2020) “Characterization of Sub-Regional Variation in Saccharomyces Populations and Grape Phenolic Composition in Pinot Noir Vineyards of a Canadian Wine Region”, Frontiers in Genetics, in press.

*these 2 authors contributed equally to this work

Cheung, S., Manhas, S. and Measday, V. (2018) “Retrotransposon targeting to RNA Polymerase III-transcribed genes”. Mobile DNA  April 23; 9:14.

Manhas, S., Ma, L. and Measday, V. (2018) “The yeast Ty1 retrotransposon requires components of the Nuclear Pore Complex for transcription and genomic integrationNucleic Acids Research 2018 Apr 20; 46(7)3552-3578.

Martiniuk, J.T., Pacheco, B., Russell, G., Tong, S., Backstrom, I. and Measday, V. (2016) “Impact of Commercial Strain Use on Saccharomyces cerevisiae Population Structure and Dynamics in Pinot Noir Vineyards and Spontaneous Fermentations of a Canadian WineryPLOS ONE, Aug 23;11(8):e0160259

Cheung, S., Ma, L., Chan, P.H., Hu, H.L., Mayor, T., Chen, H.T. and Measday, V.  (2016) “Ty1 integrase interacts with RNA Polymerase III-specific subcomplexes to promote insertion of Ty1 elements upstream of Polymerase (Pol) III-transcribed genesJournal of Biological Chemistry, Mar18; 291(12): 6396-411

Measday, V. and Stirling, P.C.  (2016) “Navigating yeast genome maintenance with functional genomicsBriefings in Functional Genomics, Mar; 15(2): 119-29

Ho, K., Ma, L., Cheung, S., Manhas, S., Fang, N., Wang, K., Young, B., Loewen, C., Mayor, T. and Measday, V. (2015) “A role for the budding yeast separase, Esp1, in Ty1 element retrotranspositionPLOS Genetics Mar 30; 11(3):e1005109

Luo, Z., Walkey, C.J., Madilao, L. L. Measday, V. and van Vuuren, H.J.J. (2013) “Functional improvement of S. cerevisiae to reduce volatile acidity in wineFEMS Yeast Research 13(5): 485-494

McQueen, J., van Dyk, D., Young, B., Loewen, C. and Measday, V. (2012) “The Mck1 GSK-3 kinase inhibits the activity of Cdk2-Cdk1 post-nuclear division.Cell Cycle 11(18); 3421-3432

Ma, L., Ho, K., Piggott, N., Luo, Z. and Measday, V. (2012) “Interactions between the Ndc80 kinetochore complex and the protein kinase A pathway in Saccharomyces cerevisiaeG3:  Genes, Genomes, Genetics 2(7): 831-41

Anderson, M. J., Barker, S. L., Boone, C. and Measday, V. (2012) “Identification of RCN1 and RSA3 as ethanol tolerant genes in Saccharomyces cerevisiae using a high copy barcoded libraryFEMS Yeast Research 12(1): 48-60

Piggott, N., Cook, M., Tyers, M. and Measday, V.  (2011) “Genome-wide fitness profiles reveal a requirement for autophagy during yeast fermentationG3:  Genes, Genomes, Genetics 1(5): 353-367

Walkey, C.J., Luo, Z., Borcher, C.H., Measday, V. and van Vuuren, H.J.  (2011) “The Saccharomyces cerevisiae fermentation stress response protein Igd1/Yfr017p regulates glycogen levels by inhibiting the glycogen debranching enzymeFEMS Yeast Research 11(6): 499-508

Ma, L., McQueen, J., Cuschieri, L., Vogel, J. and Measday, V.  (2007) “Spc24 and Stu2 promote spindle integrity when DNA replication is stalledMolecular Biology of the Cell 18(8): 2805-2816