“Structural Characterization of the Bacterial Injectisome,” by Dorothy Majewski, PhD Candidate, Strynadka Lab.
Abstract: The injectisome is a syringe-like protein complex essential for the virulence of many Gram-negative pathogenic bacteria. As a potential target for drug and vaccine design, studies to elucidate its structure have been ongoing over the past two decades. Two components of this system are essential to its function: the pore-assembling pilotin, and the cytosolic ATPase. Through X-ray crystallography, nuclear magnetic resonance, and cryo-electron microscopy, we have solved the structures of these proteins and learned key details about their interfaces with other members of the injectisome complex. Biochemical experiments have further improved our understanding of the binding and enzyme kinetics of these proteins. These structures have given us insight into injectisome assembly and function, forming a basis for future drug design.
Monday, September 14, 2020 at 2:30, join by Zoom.