Title: The last dance with peptidiscs: Characterization of membrane proteomes from prokaryotic and eukaryotic cells using peptidiscs
Abstract: Membrane proteins fulfill numerous essential roles in both prokaryotic and eukaryotic cells. However, the characterization of membrane proteomes has lagged due to their intrinsic hydrophobicity and low abundance. Although detergents can solubilize membrane proteins, they can adversely affect their stability and functionality. In this talk, I will introduce a membrane mimetic – peptidisc functionalized with a His-tag – to characterize membrane proteins. This method allows the enrichment of (plasma) membrane proteome and can be used to capture the differentially expressed proteins from different cells. Most importantly, membrane proteins captured in peptidisc can be further used in functional studies.
Membrane protein-protein interaction is another critical aspect of understanding the cellular activities of membrane proteins. Using membrane insertase YidC as a case study, I will introduce multiple proteomic-based methods to identify the interactors of YidC.
Monday, February 6, 2023 at 2:30 pm at LSC 3 and Zoom
Hosted by: Dr. Franck Duong